vitronectin (R&D Systems)

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R&D Systems vitronectin

(A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for <t>vitronectin,</t> MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

Vitronectin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin/Mouse+Clusterin+Antibody/bio_rxiv__64898__2026__03__19__712761-240-18-19
Average 93 stars, based on 45 article reviews

vitronectin - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation"

Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

Journal: bioRxiv

doi: 10.64898/2026.03.19.712761

Figure Legend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

Techniques Used: Immunofluorescence, Staining, Fluorescence, Western Blot

Cell Culture:

Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
Article Snippet: .. The 24-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 20 μg/well; 1 mL PBS/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (100,000 cells/well) and incubated overnight at 37°C.

Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
Article Snippet: HEPG2 (ATCC, HB-8065) cells were grown and maintained in T75 flasks (Corning, 430,641 U) at 37°C and 5% CO 2 in DMEM (Gibco, 11,995–065) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, A38401) and 1% penicillin/streptomycin (Gibco, 15,140,122). .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL phosphate-buffered saline (PBS)/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (350,000 cells per well) and incubated overnight.

Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
Article Snippet: .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL PBS/well) by incubating for 1 hour at 37°C. ..

Saline:

Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
Article Snippet: HEPG2 (ATCC, HB-8065) cells were grown and maintained in T75 flasks (Corning, 430,641 U) at 37°C and 5% CO 2 in DMEM (Gibco, 11,995–065) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, A38401) and 1% penicillin/streptomycin (Gibco, 15,140,122). .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL phosphate-buffered saline (PBS)/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (350,000 cells per well) and incubated overnight.

Over Expression:

Article Title: Degradomic Identification of Membrane Type 1-Matrix Metalloproteinase as an ADAMTS9 and ADAMTS20 Substrate.
Article Snippet: .. For MT1-MMP overexpression experiments, 8-chamber glass slides were coated with 5 μg/ml vitronectin (R&D systems, 2308-VN) for 2 h at room temperature prior to being seeded with parental RPE-1 cells. .. After 24 h culture, the cells were transfected with 100 ng/well of pcDNA3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765).

Article Title: Degradomic Identification of Membrane Type 1-Matrix Metalloproteinase as an ADAMTS9 and ADAMTS20 Substrate
Article Snippet: .. For MT1-MMP overexpression experiments, 8-chamber glass slides were coated with 5 μg/ml vitronectin (R&D systems, 2308-VN) for 2 h at room temperature prior to being seeded with parental RPE-1 cells. .. After 24 h culture, the cells were transfected with 100 ng/well of pcDNA3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765).

Control:

Article Title: Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells.
Article Snippet: .. A total of 96 well flat-bottom plates (Nunc Immunoplate Maxisorp, Fisher Scientific) were coated with various concentrations of extracellular matrix proteins, including fibronectin (Corning), vitronectin (2349-VN-100; R&D), collagen I (354243; Corning), and collagen IV (354233; BD Biosciences), or BSA as control. ..

Article Title: Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells
Article Snippet: .. A total of 96 well flat-bottom plates (Nunc Immunoplate Maxisorp, Fisher Scientific) were coated with various concentrations of extracellular matrix proteins, including fibronectin (Corning), vitronectin (2349-VN-100; R&D), collagen I (354243; Corning), and collagen IV (354233; BD Biosciences), or BSA as control. ..

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